| Rep. | Tranmere Rovers | Reporté | Burton Albion |
| 00h00 | Once Caldas | Huracan | |||
| 02h30 | America de Cali | Fluminense | |||
| 02h30 | Independiente del Valle | Mushuc Runa |
| 02h30 | Santa Fe | Millonarios |
| 00h00 | Fortaleza | Velez Sarsfield | |||
| 02h30 | Atletico Nacional | Sao Paulo | |||
| 02h30 | Club Atletico Penarol | Racing Club |
| FT | Chico FC | 0-0 (0-0) | La Equidad |
| FT | Grasshopper Zurich | 1-2 (0-2) | Bayern Munich | ||
| FT | WSG Tirol | 0-4 (0-2) | Real Madrid | ||
| FT | Monza | 2-2 (1-1) | Inter Milan |
| FT | Qarabag FK | 5-1 (4-1) | KF Shkendija | ||
| FT | FC Koebenhavn | 5-0 (2-0) | Malmoe FF | ||
| FT | Fenerbahce | 5-2 (2-1) | Feyenoord | ||
| FT | Pafos FC | 2-0 (1-0) | Dynamo Kyiv | ||
| FT | Viktoria Plzen | 2-1 (1-0) | Rangers | ||
| FT | Club Brugge | 3-2 (0-2) | Red Bull Salzbourg | ||
| FT | Ferencvarosi TC | 3-0 (1-0) | Ludogorets Razgrad | ||
| Pen. | Slovan Bratislava | 1-0(1-0) | Kairat Almaty* | ||
| FT | Benfica | 2-0 (2-0) | Nice | ||
| FT | FK Crvena Zvezda | 1-1 (1-0) | Lech Poznan |
| FT | Swansea City | 3-1 (1-0) | Crawley Town | ||
| FT | Barrow | 0-1 (0-0) | Preston North End | ||
| FT | Middlesbrough | 0-4 (0-2) | Doncaster Rovers | ||
| FT | Newport County | 0-1 (0-0) | Millwall | ||
| FT | Stockport County | 3-1 (0-0) | Crewe Alexandra | ||
| FT | Accrington Stanley | 2-1 (2-0) | Peterborough United | ||
| FT | Blackburn Rovers | 1-2 (1-2) | Bradford City | ||
| FT | Blackpool | 0-1 (0-0) | Port Vale | ||
| FT | Bristol City | 2-0 (1-0) | Milton Keynes Dons | ||
| FT | Bristol Rovers | 0-2 (0-1) | Cambridge United | ||
| FT | Cardiff City | 2-1 (2-0) | Swindon Town | ||
| FT | Charlton Athletic | 3-1 (2-0) | Stevenage | ||
| FT | Chesterfield | 0-2 (0-1) | Mansfield Town | ||
| FT | Coventry City | 1-0 (0-0) | Luton Town | ||
| Pen. | Gillingham | 1-1(1-1) | AFC Wimbledon* | ||
| FT | Grimsby Town | 3-1 (1-0) | Shrewsbury Town | ||
| FT | Harrogate Town | 1-3 (0-1) | Lincoln City | ||
| FT | Leyton Orient | 0-1 (0-0) | Wycombe Wanderers | ||
| FT | Northampton Town | 0-1 (0-0) | Southampton | ||
| FT | Oxford United | 1-0 (1-0) | Colchester United | ||
| FT | Plymouth Argyle | 3-2 (0-2) | Queens Park Rangers | ||
| FT | Portsmouth | 1-2 (0-2) | Reading | ||
| Pen. | Salford City | 0-0(0-0) | Rotherham United* | ||
| Pen. | *Stoke City | 0-0(0-0) | Walsall | ||
| FT | Watford | 1-2 (0-2) | Norwich City | ||
| Pen. | West Bromwich Albion | 1-1(1-1) | Derby County* | ||
| FT | Wigan Athletic | 1-0 (1-0) | Notts County | ||
| Pen. | *Wrexham | 3-3(3-3) | Hull City | ||
| Pen. | *Bromley | 1-1(1-1) | Ipswich Town |
| FT | Shelbourne | 1-3 (0-1) | Rijeka |
| FT | Sénégal | 1-1 (0-1) | Congo | ||
| FT | Soudan | 4-0 (2-0) | Nigéria |
| FT | Ruzomberok | 1-3 (0-0) | Skalica |
| FT | Leon | 1-3 (0-2) | Monterrey | ||
| FT | FC Juarez | 0-2 (0-1) | Toluca | ||
| FT | Atletico de San Luis | 1-2 (0-0) | Cruz Azul |
